1) Multiple answer. Restriction endonucleases have a number of characteristics,
ID: 47769 • Letter: 1
Question
1) Multiple answer. Restriction endonucleases have a number of characteristics, including:
a) They were primarily isolated from phage DNA, such as bacteriophage lambda.
b) They can cut palindromic DNA sequences.
c) They are used to digest a PCR product and a plasmid and with the aid of ligase, can be used to generate recombinant DNA such as for protein expression.
d) They hydrolyze recombinant DNA at the ribose base.
e) They have been primarily isolated from bacteria
2) True or false:In quantitative PCR (qPCR) the florescence is monitored in the course of a PCR amplification, do determine the CT, the cycle at which the signal exceeds a defined threshold, and is used for example to determine the levels of mRNA in a cell.
3) True or false: DNA microarrays use the Sanger dideoxy sequencing method to help monitor changes in gene expression, such as analyses yeast gene expression under a range of conditions such as heat shock, nitrogen depletion and amino acid starvation.
4) Multiple answer. When conducting PCR studies, which of the follow may occur.
a) The PCR products are isolated using a polyacrylamide gel
b) RNA-based primers are designed an used to start the polymerase reaction
c) After 30 cycles the original piece of DNA can be amplified about a billion fold.
d) The PCR products are isolated using an agarose gel
e) The PCR method can be used in forensic science to determine if a sample, such as blood is from a certain individual
Explanation / Answer
1.
Restriction endonucleases are the enzymes that are primarily isolated from bacteria and they cut DNA molecule at a specific sequence. These enzymes identify a specific palindromic DNA sequence and cut it .
b) They can cut palindromic DNA sequences.
c) They are used to digest a PCR product and a plasmid and with the aid of ligase, can be used to generate recombinant DNA such as for protein expression
e) They have been primarily isolated from bacteria
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