Use the Lineweaver-Burk approach for analysis of Michaelis -Menton kinetics for

Question

Use the Lineweaver-Burk approach for analysis of Michaelis -Menton kinetics for the following reaction. Solve for ka_2, K_M, and the maximum reaction rate, v_max, not necessarily in this order. You will need to plot this information as a straight line to extract the slope and intercept. Logger Pro, ExCEL, or graph paper should do the job. Sodium succinate oxidizes to sodium fumarate using dissolved oxygen in the presence of the enzyme succinoxidase, [E]_o = 3.0 nM. The following data was obtained from experiment.

Explanation / Answer

Lineweaver-burk plot is given by 1/V= 1/Vmax + (KM/Vmax)*1/S

so a plot of 1/V and 1/S gives a straight line whose intercept is 1/Vmax and slope is KM/Vmax

the plot is shown below

the intercept is 1/Vmax= 0.000008, Vmax= 1/0.000008=1.25*106 M/s

K2[E]= 1.25*106, K2= 1.25*106/3*10-9 M=4.17*1014/S, KM/Vmax= slope = 8*10-7, Km= 8*10-7*1.25*106 =1M

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