Accurately weigh (to 0.1 mg) about 0.1g of pure salicylic acid (weight by differ

Question

Accurately weigh (to 0.1 mg) about 0.1g of pure salicylic acid (weight by difference using a weighing bottle) and quantitatively transfer to a 100 ml volumetric flask. After thorough mixing dilute to the mark with a NaOH solution so that the final concentration is 0.1 N NaOH. Calculate the salicylic acid concentration (mg/L) in this Stock solution. Prepare five dilutions of your stock solution using 1,2,3,4, and 5 mls diluted to 100ml with 0.1 N NaOH. Calculate the salicylic acid concentration (mg/L) in your stock solution and each standard

Explanation / Answer

0.1 g salicyclic acid = 100 mg

100 mL = 0.1 L

Stock solution concentration (mg/ L) = 100 mg/ 100 mL * 1000 ml / 1L = 1000 mg/ L

For preparation of dilute solutions if 1 ml stock solution is taken, it contains 1 mg protein.

Stock concentration -1000 mg/ L Dilute solution no. Weight of protein (mg) Final volume (mL) Concentration (mg/ L) 1 1 100 10 2 2 100 20 3 3 100 30 4 4 100 40 5 5 100 50

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