weight of the you will be detecting is 600yftow many mmoles of the sugar of 30 m
ID: 474819 • Letter: W
Question
weight of the you will be detecting is 600yftow many mmoles of the sugar of 30 ml, and the molecular be in the experimental solution when the detection limit is reached? Problem #2: You have been hired as an orderly in an Emergency Room at local hospital. Your first night on duty a comatose patient wheeled in. Blood is analysis readily reveals the problem: instead of the normal serum potassium concentration of 5 mM, your patient has a concentration of 1.5 mM. Since thi such an easy problem to rectify, the attending physician gives you a tube of KCI instructing you to add the necessary amount to the patient's IV drip, and hurries off to more pressing concerns. Looking at the tube in your you notice the label "2M". hand, Recalling that the combined volume of blood plus interstitial fluid in an average person is about 15 L, how much of the KCI solution should you add to bring the patient back to normal potassium levels? Problem #3: Since you did such a great job with the last patient, the attending physician asks you to help out with a stabbing victim. They've stopped the bleeding, but the patient has lost 1.0 L of blood and is going into hypovolemic shock. Doc wants you to give the patient normal saline until the blood type match comes back, but it's been so hectic that you've run out! All you have is sterile water and sodium chloride, with the notation: "Molecular weight 58.5 Remembering that normal body osmolarity is 300 mosM, how much Naci should you add to the 1.0 L of water for your patient? Problem #4: Slightly frazzled from your experience in the ER, you decide to try your hand at research. In just a few weeks, you discover an enzymatic reaction that produces a new drug given the name Panacein. Excited with the results of initial trials, the head of the lab calls a colleague to help with structural analysis of the new drug. The structural chemist says that she will need at least 20 mg of Panacein for her studies. You only have 50 ug of the enzyme left, and the enzyme has a specific activity of 1.5 umol product formed/mg enzyme/min. lf the molecular weight of ein is 1400, how long will it take to synthesizeExplanation / Answer
Let t minutes be the time required.
The enzyme has an activity of 1.5 µmol product formed/mg enzyme/min.
We have 50 µg enzyme left. Therefore, mass of enzyme left in mg = (50 µg)*(1 g/106 µg)*(1000 mg/1 g) = 0.05 mg.
Therefore, moles of product produced = (1.5 µmol product/mg enzyme/min)*(0.05 mg)*(t min) = 0.075t µmole.
Again, 20 mg Panacein is required and the molar mass of Panacein is 1400 g/mol. Therefore, moles of Panacein required = (20 mg)*(1 g/1000 mg)*(1 mole/1400 g) = 1.4286*10-5 mole = (1.4286*10-5 mole)*(1 µmole/10-6 mole) = 14.286 µmole.
Therefore, as per the problem,
0.075t µmole = 14.286 µmole
===> t = 14.286/0.075 = 190.48
The time required = 190.48 minutes = (190.48 min)*(1 hour/60 min) = 3.175 hour (ans).
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