You have partially sequenced the protein from a kangaroo. The sequence is Leu Me

Question

You have partially sequenced the protein from a kangaroo. The sequence is Leu Met Asp Cys Trp Ile Thr Phe Ile. You want to extract the corresponding gene from wombats which you believe has the same amino acid sequence as shown. To identify the gene you want to synthesise an oligonucleotide of 15 bases for use as a probe. What region of the peptide would you use to develop your oligo using the least number of combinations but giving a sequence that must be absolutely complementary to your corresponding wombat sequence?

Explanation / Answer

The portion of peptide to be used to develop oligo would be Leu Met Asp Cys Trp. This is because of the least number of combinations that would be used:

AAUUACCCAACAAAC

This would be complementary to

UUAAUGGGUUGUUGG

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