Which of the following steps are NOT done for preparing aslide from a liquid sus

Question

Which of the following steps are NOT done for preparing aslide from a liquid suspension of bacteria? [Check ALL that are NOTdone]



a. Transfer several "loopfulls" ofbacteria to slide

b. Add a 1/2 drop ofdistilled water to slide.

c. Spread sample over slideand fix.

d. Flame loop beforeinsertion into tube.

e. Use a coverslip to holdbacteria in place.
The best way to prepare samples to observe the organization ofbacteria clusters is by



a. indirect staining with a negativelycharged dye.

b. Direct staining with apositively charged dye.

c. Differential staining withGram's stain.

d. -- All of these stains givean equally good visualization of structure.
Which of the following steps are NOT done for preparing aslide from a liquid suspension of bacteria? [Check ALL that are NOTdone]



a. Transfer several "loopfulls" ofbacteria to slide

b. Add a 1/2 drop ofdistilled water to slide.

c. Spread sample over slideand fix.

d. Flame loop beforeinsertion into tube.

e. Use a coverslip to holdbacteria in place.
The best way to prepare samples to observe the organization ofbacteria clusters is by



a. indirect staining with a negativelycharged dye.

b. Direct staining with apositively charged dye.

c. Differential staining withGram's stain.

d. -- All of these stains givean equally good visualization of structure.

Explanation / Answer

      1.) b. Add a 1/2 drop of distilled water toslide.                     Asthe slide is prepared form a suspension of liquid bacteria, thereis no requirement of water.            a. Transferseveral "loopfulls" of bacteria to slide                      Asingle loop of culture is required but not several loopfulls.

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