My answers are as follows... A, C, B, A. Am I correct? Multiple choice: 1. Which

Question

My answers are as follows... A, C, B, A. Am I correct?

Multiple choice:

1. Which one of the following is NOT involved in regulating the function of mitotic cyclin dependent kinase (mitotic cdk)?

a. Regulating when mitotic CDK protein is made

b. Regulating the state of phosphorylation of the mitotic CDK

c. Regulating whether it is bound by cyclin or not

d. Regulating whether it is bound by p27 protein or not

2. Choose the correct sequence of activities to clone (identify) gene X using a mutant, when gene X is not yet identified.

a. Generate a DNA library of the wild type version of the species, introduce the selectable library into the mutant strain, screen for complementation of the mutant phenotype

b. Generate a recombinant vector that contains gene X specifically, introduce the selectable recombinant vector into mutant strain, screen for complementation of the mutant phenotype

c. Generate a DNA library of the mutant version of the species, introduce the selectable library into the mutant strain, screen for complementation of the mutant phenotype.

d. Generate a DNA library of the wild type version of the species, introduce the selectable library into the wild type strain, screen for complementation of the mutant phenotype.

3. In experiments of Xenopus laevis oocyte extracts directed at the regulation of mitotic cdk/cyclin (MFP), what result would you predict if a proteasome inhibitor drug was added to the sample after S phase.

a. MPF activity will remain high and will not oscillate

b. The cells will remain stuck in early mitotic phase

c. Cyclin concentration will never rise to detectible levels

d. Both A and B

4. Which one of the following is NOT a substrate of APC?

a. Lamin proteins

b. Mitotic cyclin

c. Securin

d. Both B and C

Explanation / Answer

1. OPTION A

2. OPTION D

3. OPTION D

4. OPTION A

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