A microbiologist was studying a particular Baculovirus and routinely grew this v
ID: 43561 • Letter: A
Question
A microbiologist was studying a particular Baculovirus and routinely grew this virus in Sf21 cells. He had a stock suspension of this virus which he had previously determined to contain 6.2 x 108plaque-forming units per ml. In a particular experiment one day, he removed 100 microliters from his Baculovirus stock solution and diluted this into 9.9 ml diluent. He then removed a 20 microliter aliquot from this dilution tube and added it to a well with Sf21 cells.
The dose of virions that the Sf21 culture received was...
Explanation / Answer
Dilution was made from 100 microlitre ( 0.1 ml) to 9.9 ml , which will in total account to 100 times dilution of the stock solution.
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