EDOVO-KIT #301 Study Questions 2. Which of the following pairs could be ligated

Question

EDOVO-KIT #301

Study Questions

2. Which of the following pairs could be ligated together? (All termini are cohesive and complementary.)

5'-dephosphorylated linear insert DNA + linear vector

Supercoiled vector + linear insert DNA

5'-dephosphorylated linear vector + linear

5'-dephosphorylated insert DNA

Linear 5'-dephosphorylated vector + linear insert DNA

Nicked vector + linear insert DNA

In general, which of the above possibilities would be the best approach in a subcloning experiment like the one you have done? Why?

Explanation / Answer

The enzyme in the ligation reaction catalyzes the formation of phosphodiester bond by condensation of 5

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