PART B: I -d P + O + Z + Y + PART C: crp - I -d P + O + Z + Y + Part D: I - P +

Question

PART B: I-dP+O+Z+Y+
PART C: crp- I-dP+O+Z+Y+
Part D: I-P+OCZ+Y-/I+P+O+Z-Y+
PART E: I+P-OCZ+Y-/I+P+O+Z-Y+
PART F: I-P+OCZ-Y+/I+P+O+Z+Y-
PART G: I+P+O+Z-Y+/I-P+O+Z+Y-
PART H: I+P+OCZ+Y+/ISP+O+Z-Y-

ALL OF THESE ARE ANSWERED THE SAME WAY AS THE ONE ON THE PICTURE

For this genotype, indicate whether the enzyme is indicated is produced at significant levels. beta galactosidase. no lactose present beta galactosidase, lactose present as sole carbon source permease, no lactose present permease, lactose present as sole carbon source To enter your answer, list only the numbers of the situations that would produce the enzyme at significant levels. Write them in numerical order without spaces or any other characters. If none of the situations would produce significant enzyme production, write a single 0. For example: 124

Explanation / Answer

In E. coli, lacZ gene (?-galactosidase gene) is present as part of the inducible system lacoperon. This gene is expressed and ?-galactosidase is synthesised when glucose levels are low and lactose is present as carbon source. Permease transports lactose across cell membrane, in the presence of latose, permease is also synthesised (lacY gene).

Thus, the correct options are 2 and 4 (as you require, it is 24).

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