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I S P + O C Z + Y + For this genotype, indicate whether the enzyme is indicated

ID: 42606 • Letter: I

Question

ISP+OCZ+Y+

For this genotype, indicate whether the enzyme is indicated is produced at significant levels.

1 ?-galactosidase, no lactose present

2 ?-galactosidase, lactose present as sole carbon source

3 permease, no lactose present

4 permease, lactose present as sole carbon source

To enter your answer, list only the numbers of the situations that would produce the enzyme at significant levels.

I-dP+O+Z+Y+

For this genotype, indicate whether the enzyme is indicated is produced at significant levels.

1 ?-galactosidase, no lactose present

2 ?-galactosidase, lactose present as sole carbon source

3 permease, no lactose present

4 permease, lactose present as sole carbon source

crp- I-dP+O+Z+Y+

For this genotype, indicate whether the enzyme is indicated is produced at significant levels.

1 ?-galactosidase, no lactose present

2 ?-galactosidase, lactose present as sole carbon source

3 permease, no lactose present

4 permease, lactose present as sole carbon source

I-P+OCZ+Y-/I+P+O+Z-Y+

For this genotype, indicate whether the enzyme is indicated is produced at significant levels.

1 ?-galactosidase, no lactose present

2 ?-galactosidase, lactose present as sole carbon source

3 permease, no lactose present

4 permease, lactose present as sole carbon source

I+P-OCZ+Y-/I+P+O+Z-Y+

For this genotype, indicate whether the enzyme is indicated is produced at significant levels.

1 ?-galactosidase, no lactose present

2 ?-galactosidase, lactose present as sole carbon source

3 permease, no lactose present

4 permease, lactose present as sole carbon source

I-P+OCZ-Y+/I+P+O+Z+Y-

For this genotype, indicate whether the enzyme is indicated is produced at significant levels.

1 ?-galactosidase, no lactose present

2 ?-galactosidase, lactose present as sole carbon source

3 permease, no lactose present

4 permease, lactose present as sole carbon source

I+P+O+Z-Y+/I-P+O+Z+Y-

For this genotype, indicate whether the enzyme is indicated is produced at significant levels.

1 ?-galactosidase, no lactose present

2 ?-galactosidase, lactose present as sole carbon source

3 permease, no lactose present

4 permease, lactose present as sole carbon source

I+P+OCZ+Y+/ISP+O+Z-Y-

For this genotype, indicate whether the enzyme is indicated is produced at significant levels.

1 ?-galactosidase, no lactose present

2 ?-galactosidase, lactose present as sole carbon source

3 permease, no lactose present

4 permease, lactose present as sole carbon source

Explain your answer for Rating.

Explanation / Answer

1. ISP+OCZ+Y+ The option is 1. This genotype allows the formation of the enzyme beta galactosidase. Since there is a super repressor and a constitutive operator, the operator wins to allow transcription of enzymes in the presence or absence of lactose.

2. I-dP+O+Z+Y+ .The option is 1. This genotype shows the absence of the repressor. This allows the continuous expression of these genes even in the absence of lactose.

3. crp- I-dP+O+Z+Y+ The option is 1. This genotype shows the absence of the crp gene which indicates the absence of the positive regulation by the cAMP system. The negative regulation is also absent as repressor is absent.This allows the continuous expression of these genes even in the absence of lactose.

4. I-P+OCZ+Y-/I+P+O+Z-Y+ The option is 1. This genotype shows the diploid genotype. As the operator is constitutive, this blocks the action of the repressor. This allows the continuous expression of these genes even in the absence of lactose.

5. I+P-OCZ+Y-/I+P+O+Z-Y+ The option is 1. This genotype shows the diploid genotype. As the operator is constitutive, this blocks the action of the repressor. This allows the continuous expression of these genes even in the absence of lactose.

6.I-P+OCZ-Y+/I+P+O+Z+Y- The option is 1. This genotype shows the diploid genotype. As the operator is constitutive, this blocks the action of the repressor. This allows the continuous expression of these genes even in the absence of lactose.

7. I+P+O+Z-Y+/I-P+O+Z+Y- The option is 2. This genotype shows the diploid genotype. The represor protein is produced from one of the chromosomes. Hence, the enzyme is produced only in the presence of lactose.

8. I+P+OCZ+Y+/ISP+O+Z-Y-The option is 1. Since there is a super repressor and a constitutive operator, the operator wins to allow transcription of enzymes in the presence or absence of lactose.