You are given a short fragment of DNA to sequence. You clone the fragment, isola

Question

You are given a short fragment of DNA to sequence. You clone the fragment, isolate the cloned DNA fragment and set up a series of four dideoxy reactions. You then separate the products of the reations and obtain the following bands results.

Write out the base sequence that is added directly after the primer. In order for Moodle to correctly grade this question, write only the letters representing the bases, write your answer in 5' to 3' direction, and do not have any spaces between the letters and do not label the 5' and 3' ends.

5'______________3'

Explanation / Answer

Dideoxynucleotides causes chain termination. Of the four nucleotides, each one is added at each step. A dark band indicates DNA fragment that is the result of chain termination after incorporation of a each dideoxynucleotide (ddATP, ddGTP, ddCTP, or ddTTP). The DNA sequence then can be read base on the relative positions of the different bands from bottom to top.

The DNA bases in the given bands indicate,

A

C

G

T

C

A

T

G

G

C

A.

The DNA sequence is read from bottom to top, Thus, the DNA sequence represented by the given picture is, ACGGTACTGCA.

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