Superoxide dismutase (SOD) was denatured by heating in buffered SDS plus 2-merca
ID: 41981 • Letter: S
Question
Superoxide dismutase (SOD) was denatured by heating in buffered SDS plus 2-mercaptoethanol and electrophoresed in an acrylamide gel also containing SDS. Proteins of known molecular weight were analyzed in a separate lane to calibrate the gel. The bromophenol blue tracking dye traveled 80 mm. Calculate the subunit molecular weight of SOD using data in the table.
Protein
Subunit mol. wt.
Migration (mm)
Catalase
60,000
10
Ovalbumin
43,000
20
Trypsinogen
23,600
40
Cytochrome c
13,400
60
Superoxide dismutase
--------
53
Protein
Subunit mol. wt.
Migration (mm)
Catalase
60,000
10
Ovalbumin
43,000
20
Trypsinogen
23,600
40
Cytochrome c
13,400
60
Superoxide dismutase
--------
53
Explanation / Answer
Using a calibration curve we can calculate subunit molecular weight of SOD
firstly we have to ?Determine the log10 of the M.W of the proteins that are in the table
secondaly , Graph the data .
Thirdly , The log10 of the M.W is graphed on the Y axis and the distance traveled on the X axis.
fourthly , Plan the increments on each axis to distribute the variables on the graph as much as possible.
fifthly , join the points with the straight line you can give with these data. line should pass from the origin.
sixthly , determine the log10 of the M.W of the protein given the distance traveled using this line.
seventhaly ,M.W of each protein by finding the antilog of these value is calculated.
?eightly , determine your percent error looking up the molecular weight(M.W).
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.