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Use the information below to relate the efficiency and affinity of the enzymes A

ID: 41554 • Letter: U

Question

Use the information below to relate the efficiency and affinity of the enzymes A and B, which catalyze the same reaction.

Enzyme A has a Km = 100 mM and a kcat = 1 million per sec.

Enzyme B has a Km = 1M and kcat = 1 billion per sec

Use the information below to relate the efficiency and affinity of the enzymes A and B, which catalyze the same reaction.

Enzyme A has a Km = 100 mM and a kcat = 1 million per sec.

Enzyme B has a Km = 1M and kcat = 1 billion per sec

Enzyme A is more efficient Enzyme B is more efficient Enzyme A has lower affinity for substrate Both (1) and (3) Both (2) and (3)

Explanation / Answer

Kcat / Km value is used to calculate the enzyme efficincy. Kcat indicates the turnover rate. I.e. the number of substrate molecules catalysed within a sec. (1000 millions = 1 Billion).

Km indicates the enzyme efficiency towards a substrate. The lower the Km value, the heigher the enzyme efficincy. So, enzyme A has heighr affinity towards its substrate.

Thus, the option B is correct. Enzyme B is more efficient.

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