Help me with chemotaxis assay lab We did a chemotaxi assay - using these 4 mediu

Question

Help me with chemotaxis assay lab

We did a chemotaxi assay - using these 4 mediums

control, IL-8, IL-8+ Ly29004 and IL-8 + Cytochalasin D

so far i know- control will have highest number of cells and signaling.

Ly29004 is an inhibitor of p13 kinase and Cytochalasin D inhibit actin polymerization by binding to actin subunits and preventing their association and dissociation.

So my question is which will result in low signaling,, or which will have low number of cells among Ly29004 and Cytochalasin D.

My results are showing that Cytochalasin D have low cell counts .. so is this the one that inhibit signal transduction the most?? or its Ly29004

Explanation / Answer

IL-8 (Interleukin -8) induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived.

Ly29004 , as given is inhibitor of pI3 kinase (phosphatidyl inositol-3-kinases); this is a family of intracellular signal transducer enzymes involved cell growth, proliferation, differentiation, motility, survival and intracellular trafficking, which in turn are involved in cancer.

Cyto chalasin D inhibits only actin polymerisation, but will have no effect on phosphorytion and many other signal transduction pathways not involving actin.

So, the third medium, IL-8+ Ly29004 will result in low signaling

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