In some of the molecules, the low fluorescence state had a fluorescence that was
ID: 41037 • Letter: I
Question
In some of the molecules, the low fluorescence state had a fluorescence that was 56% of the fluorescence of the high fluorescence state, while in others, the low fluorescence state had a fluorescence that was 36% of the fluorescence of the low-fluorescence state. We had some results that showed rate constants for the transitions between the high and low-fluorescence states. What is the average dwell time for the molecule in the high and low-fluorescence states?
Start State End State k, -ATP Dwell time -ATP k,+1mMATP Dwell time + 1mM ATP 1.00 0.36 4.70 s-1 0.21 s 4.50 s-1 0.22 s 0.36 1.00 3.60 s-1 0.28 s 1.68 s-1 0.60 s 1.00 0.56 0.67 s -1 1.50 s 1.01 s-1 0.99 s 0.56 1.00 1.85 s-1 0.54 s 0.76 s-1 1.31 sExplanation / Answer
The average dwell time for high flourescence state (56%) is = dwelltime - ATP + Dwell time + 1mM ATP /2
= 1.50 + 0.99 /2 = 1.25 S
The average dwell time for low flourescence state (36%) is = 0.21 + 0.22 /2 = 0.215 S.
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