Below are described the phenotypes of 4 different mutant strains of E. Coli. Eac
ID: 40730 • Letter: B
Question
Below are described the phenotypes of 4 different mutant strains of E. Coli. Each strain carries only one mutation, what could the genotype of each strain be?
Strain 1: Grows on minimal medium with lactose as a sole source of carbon; mkaes blue colonies on minimal medium sucrose X-gal.
Strain 2: Does not grow on minimal medium with lactose as a sole source of carbon; makes white colonies on minimal medium sucrose X-gal IPTG; an antibody that specifically recognizes the B-gal is able to detect a protein in this strain when grown on minimal medium sucrose IPTG X-gal; an antibody that specifically recognizes the lactose permease is able to detect a protein in this strain when grown on minimal medium sucrose IPTG X-gal.
Strain 3: Makes white colonies on minimal medium sucrose X-gal IPTG; makes white colonies on minimal medium sucrose X-gal; the antibody specific for the B-Gal never detects anything.
Strain 4: Makes small colonies on minimal medium lactose; makes colonies that are of lighter blue than usual on minimal medium sucrose IPTG X-gal and on minimal medium glucose IPTG X-gal.
Explanation / Answer
Answer:
Strain 1:vibrio vulnificus.
Strain 2: Salmonella typhimurium LT-2.
Strain 3: Adenovirus.
Strain 4: M15 strain of E. coli.
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