3. When studying electron transport and oxidative phosphorylation in E. coil tha

Question

3. When studying electron transport and oxidative phosphorylation in E. coil that are growing on glucose as their sole carbon source, you add malonate, an inhibitor of succinate dehydrogenase, completely deactivating it. How do you expect this to affect the number of protons transported into the periplasmic space per glucose that is fully oxidized to CO2? How will it affect the number of ATP produced per glucose that is oxidized completely to CO2? You can assume that there is sufficient oxaloacetate so that no pyruvate needs to be used in anaplerotic reactions.

Explanation / Answer

succinate dehydrogenase complex(complex II) in ETC tranfers electrons from its FAD component and then transfers electrons to ETF:Ubiquinone oxidoreductase. (ETF is electron transferring flavoprotein) from this it is transferred to ubiquinone (Q) to form QH2 i.e. ubiquinol. this transfer of electrons from succinate dehydrogenase to Q does not transport any protons into the periplasmic space.

Thus, inhibition of complex II by malonate will not affect proton generation.

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