The following is part of a mRNA from a gene with two exons and the intervening i
ID: 40283 • Letter: T
Question
The following is part of a mRNA from a gene with two exons and the intervening intron. The upper case nucleotides indicate the nucleotides that are recognized during splicing of the intron.
5' ...caugauaaGUagcgcu...guuccAccguu...uucugcAGauuucaugau...3'
Make a simple line diagram of the lariat structure of the discarded intron using a line for most of the RNA strand and showing the loccation of all 5 nucleotides that are in bold tpeface above. label any loose ends of the structure (3' or 5').
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Explanation / Answer
mRNA: 5' ...caugauaaGUagcgcu...guuccAccguu...uucugcAGauuucaugau...3'
mRNA after splicing: 5' ...caugauaa-3` 5`--agcgcu.3` 5`..guucc-ccguu..3` 5`.uucugc-3` 5`-auuucaugau...3'
The removed 5 nucleotides: GUAAG
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