The following data was collected from DNA isolated from twodifferent bacterial c

Question

The following data was collected from DNA isolated from twodifferent bacterial cultures.
Bacterial Species Tm (0C) Adenine content
(% base content) Cytosine content
(% base content) 1 65 41 2 88 16
a. Complete this table by determining the percentage contentof the corresponding complementary bases for each bacterialpopulation. On what assumptions have you based yourcalculations?
b. Clearly explain the relationship between Tm andbase pair content of each bacterial species.
c. Explain which bacterial species would be able to survive attemperatures lower than 15 oC
Bacterial Species Tm (0C) Adenine content
(% base content) Cytosine content
(% base content) 1 65 41 2 88 16
a. Complete this table by determining the percentage contentof the corresponding complementary bases for each bacterialpopulation. On what assumptions have you based yourcalculations?
b. Clearly explain the relationship between Tm andbase pair content of each bacterial species.
c. Explain which bacterial species would be able to survive attemperatures lower than 15 oC Bacterial Species Tm (0C) Adenine content
(% base content) Cytosine content
(% base content) 1 65 41 2 88 16

Explanation / Answer

% of Adenine is 41%.So according to Chargaff's rule %of A=% ofT Therefore % of A=41    %of T=41 As they are present in % it is taken as for 100. Therefore 41+41 =82. 100-82=18 Therefore % of G=18/2 = 9% % of C = 9%. Therefore % of A=41 % of T=41 % of G = 9 % of C =9 % of A=16 % of T=16 % of G,C=100-32=68. % of G-34 % of C=34
The Tm of a species is directly related to the base pair content. When GC content is high the Tm is high. When GC content is low, the Tm is low. The stability of these bonds is due to stacking interactionsand this raises the Tm.Even the triple bond raises the Tm. The first bacterium would be able to survive at temperatureslower than 15 degrees. This is because the lower the Tm the easier the bacteria canwithstand low temperatures. The other bacterium has a higher Tm and consequently cannotwithstand lower temperatures When GC content is high the Tm is high. When GC content is low, the Tm is low. The stability of these bonds is due to stacking interactionsand this raises the Tm.Even the triple bond raises the Tm. The first bacterium would be able to survive at temperatureslower than 15 degrees. This is because the lower the Tm the easier the bacteria canwithstand low temperatures. The other bacterium has a higher Tm and consequently cannotwithstand lower temperatures The first bacterium would be able to survive at temperatureslower than 15 degrees. This is because the lower the Tm the easier the bacteria canwithstand low temperatures. The other bacterium has a higher Tm and consequently cannotwithstand lower temperatures

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