Buffer dilutions and concentrations. Detailed answers are encouraged, thanks 2.

Question

Buffer dilutions and concentrations.

Detailed answers are encouraged, thanks

2. You measure the protein concentration in your cell lysale and get the concentrations in the table below. Sample Mug/mL 1 1176 2 811 3 898 4 653 You use a 4X sample buffer to prepare your samples for SDS-PAGE. You mix such that the final concentration of the sample buffer is 1X. ie. 33Mul of 4X buffer for every 100 Mul of cell lysate. You want to load 15 Mug of protein in each well, what volume you dispense into the well of the gel to get 15 Mug? 3. You are given 100mL of a 10x Running Buffer stock solution. You need a 1X solution of Running Buffer to run your gel. How much water would you add to the stock solution?

Explanation / Answer

1- 1176ug in 1000ul.(1000/1176)*15=12.7ul

2- 18.4ul ; 3. 16.7ul ; 4. 22.9ul

3. 100x to 1x. dilute 100 times. take 1ml of 100x and add 99ml water to make final 100ml of 1x

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