You have just read a paper that the addition of caffeine to cells disrupts the D

Question

You have just read a paper that the addition of caffeine to cells disrupts the DNA damage checkpoint and you would like to understand why. By searching for proteins that bind to caffeine, you isolate a protein, Caf1. You believe that Caf1 is involved in the DNA damage checkpoint because when you delete Caf1 from yeast cells and add a drug that causes DNA damage, the cells fail to arrest in the cell cycle.

You give your Caf1-deleted cells to an undergraduate in the laboratory and ask him to take care of the strain for you while you take the weekend off to celebrate your finding. Upon your return, you find the poor undergraduate in tears. He explains that he thinks that he messed up the Caf1 mutant strain while you were gone because after growing Caf1 and wild-type cells in rich media and examining them during mitosis, Caf1 mutants looked the same as wild-type cells. Are you concerned about the undergraduate student

Explanation / Answer

yes..

On addition of drug that causes DNA damage to the CAF1 deleted cells (resonsible for DNA damage check point ) there is no arrest of cell cycle which simly means that the cell will divide rapidly more than the normals cells.

in case of wild type of CAF1 cells there will be normal cell division.because it will stoop the cell division at DNA damage checkpoint, both cells will look different.

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