The lysozyme coding region can be inserted into the pBNJ vector in two possible

Question

The lysozyme coding region can be inserted into the pBNJ vector in two possible orientations. Determine the base pair position (based on the orientation of the insert) of each of the following sites in the recombinant plasmid maps:
Insert Orientation
Restriction Enzyme w/in Insert
Recombinant Plasmid Position
Native   HindIII   Answer
Native   XhoI   Answer
Non-native   HindIII   Answer
Non-native   XhoI   Answer

The lysozyme coding region can be inserted into the pBNJ vector in two possible orientations. Determine the base pair position (based on the orientation of the insert) of each of the following sites in the recombinant plasmid maps: Insert Orientation Restriction Enzyme w/in Insert Recombinant Plasmid Position Native HindIII Answer Native XhoI Answer Non-native HindIII Answer Non-native XhoI Answer

Explanation / Answer

non -native insert HindIII orientation is reversed.

non -native insert XhoI orientation is reversed.

rest two orientation is in same direction as plasmid.

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