35 - Osmosis 36- Abbe Nollet 37 - RB( Bursting 38 - Colligative Phenomena 1. Dia

Question

35 - Osmosis 36- Abbe Nollet 37 - RB( Bursting 38 - Colligative Phenomena 1. Dialysis is a process used to remove small unwanted contaminants from protein isolations. Dialysis works by placing the sample of interest in a ?dialysis bag? (which is basically a bag made from a semipermeable membrane that allows small molecules to pass while retaining large molecules) and then placing the dialysis bag in a buffer solution with 100-200 times more volume. By what factor will the concentration of unwanted small molecules be reduced if a bag with 10 mL protein solution is placed in 1000 mL (Develop general equation first.)

Explanation / Answer

35. Osmosis is the spontaneous net movement of solvent molecules through a semi-permeable membrane into a region of higher solute concentration, in the direction that tends to equalize the solute concentrations on the two sides.

36) Jean-Antoine Nollet (19 November 1700

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