I am using a RNA which is in vitro transcripted before I started my project. It

Question

I am using a RNA which is in vitro transcripted before I started my project. It turned out it is not prepared properly and has DNA contamination.

Instead of perform the in vitro transcription again, I want to try to clean it with DNase treatment and LiCl precipitation afterwards. Do you think would that work?

And second thing, in vitro transcipted RNA is in different dilutions, so I have a little bit of stock (maybe 5 uL) which is around 200 ng/uL and I have other dilutions 1 and 0.1 ng/uL. I would like to clean all of them. Do you think if I just mix all of the dilutions with the stock and determine the concentration and do the rest? Is it appropriate?

Thanks!

Explanation / Answer

If you know, your the contamination happened after the transcription, DNase treatment should obviously help.

Concerning the aliquots: your stock is 200 times more concentrated than the aliquots (so to get equal amounts of RNA as in 1

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