Suppose that you accidentally added 10-fold more ddGTP to the reaction in lane 4

Question

Suppose that you accidentally added 10-fold more ddGTP to the reaction in lane 4. What effect would that have on the banding pattern in lane 4?

You decide to perform dideoxy sequencing on a PCR product. You add the appropriate 32P-labeled primer, DNA polymerase, DNA template (the PCR product), buffer, dNTP mix, and a small amount of one of the four ddNTPs to four reaction tubes. You run the reactions in the thermal cycler, load each reaction into a separate lane of a polyacrylamide gel, and separate the products by gel electrophoresis. In the figure below, the lanes are labeled according to the ddNTP added. What is the sequence of the template strand? Suppose that you accidentally added 10-fold more ddGTP to the reaction in lane 4. What effect would that have on the banding pattern in lane 4?

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