You find a transcription activator called Msn2 in yeast. You do a deletion analy
ID: 35526 • Letter: Y
Question
You find a transcription activator called Msn2 in yeast. You do a deletion analysis to dissect out the function of each part of the protein. When you delete the N-terminus you get no DNA binding or transcription of a reporter for Msn2 activity. When you delete the C-terminus, you get DNA binding but no transcription. You get lots of reporter activity with the wild type protein with no deletion. Which of the following statements is true?
The reporter you are using isn't activated by Msn2.
You only need the the activation domain for this transcriptional activator to work.
The N-terminus contains the activation domain of the transcriptional activator
The N-terminus contains the DNA binding domain of the transcriptional activator
Which of the following is a ribozyme?
initiator tRNA
small subunit of ribosome
eRF1
large subunit of the ribosome
Which of the following is not involved in mRNA degradation?
deadenylation
decapping
CTD phosphorylation
exonucleases
The reporter you are using isn't activated by Msn2.
You only need the the activation domain for this transcriptional activator to work.
The N-terminus contains the activation domain of the transcriptional activator
The N-terminus contains the DNA binding domain of the transcriptional activator
Explanation / Answer
1. The reporter you are using isn't activated by Msn2.
2. large subunit of the ribosome
3. CTD phosphorylation
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