Temperature-sensitive mutations are useful in understanding essential life proce
ID: 35280 • Letter: T
Question
Temperature-sensitive mutations are useful in understanding essential life processes. A temperature-sensitive mutant will be normal at a lower (non-restrictive) temperature; when shifted to a higher (restrictive) temperature, the mutant will reveal its mutant (and potentially deleterious) phenotype. You isolate a plant with a temperature-sensitive mutation in the water-splitting complex. You grow the plant at a non-restrictive temperature, then shift it to its restrictive temperature. The outcome you would most expect to see upon shifting temperature would be
decreased NADPH production. increased NADPH production. no change in NADPH production. decreased sugar production. no change in sugar production. increased oxygen production.Explanation / Answer
Temperature sensitive mutants:
Temperature-sensitive mutations are powerful tools for studying the gene function.
Temperature-sensitive alleles are rare and difficult to generate and identify, and this has limited their use in most multicellular organisms.
Since, the proteins are effected by increase in temperature, i:e. proteins are denatured by high temperatures it can be assumed that NADPH production decreases because NADPH is produced by enzyme catalysed reactions and also the sugar production will decrease and the oxygen production will not increase but would decrease due to the above mentioned reason again. Therefore correct answers are
1.decrease in NADPH production
2.decrease in sugar production
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