You have a solution containing the following proteins Protein pI Mol. Weight, kD
ID: 35275 • Letter: Y
Question
You have a solution containing the following proteins
Protein
pI
Mol. Weight, kDa
Protein 1
4.2
125
Protein 2
5.7
32
Protein 3
9.6
10
Protein 4
3.2
45
Protein 5
12.0
60
1) You load this solution onto a DEAE anion exchange column at pH 5.7. Which proteins bind to your column? Briefly explain your answer.
2) You now load these same proteins onto a carboxymethyl cation exchange column at pH 7. Please list the proteins that do not bind to your column and appear in the effluent.
3) You load this same mixture onto a size exclusion column. Please indicate the order of elution.
Protein
pI
Mol. Weight, kDa
Protein 1
4.2
125
Protein 2
5.7
32
Protein 3
9.6
10
Protein 4
3.2
45
Protein 5
12.0
60
Explanation / Answer
1) Protein 3
2) Protein 2, 4
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