When adding your DNA samples to the agarose gel, you will need to add loading dy

Question

When adding your DNA samples to the agarose gel, you will need to add loading dye to the samples. Loading dye contains:

glycerol, which helps the samples sink into the sample wells of the gel; and

tracking dyes so that you have a way of estimating how far the samples have travelled through the gel during electrophoresis.

The recipe for 6X loading dye is as follows:

0.25% bromophenol blue (w/v)

0.25% xylene cyanol (w/v)

30% glycerol in water (v/v)

11. You will be asked to make 20 ml of 10X loading dye. Using the information above, provide a recipe.

Explanation / Answer

. Dissolve in 6.25 ml of H2O

           .025g of Xylene cyanol

           .025g of Bromophenol Blue

         1.25ml of 10% SDS

         12.5ml of glycerol

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