The hydrolysis of glucose-6-phosphate to glucose and phosphate is an important s
ID: 35234 • Letter: T
Question
The hydrolysis of glucose-6-phosphate to glucose and phosphate is an important step in glycogen catabolism. The free glucose formed by this reaction is released intto the blood for transport to cells in need of energy
glucose-6-phosphate +H20----> Glucose/phosphate
b. In which direction would you expect the reaction to proceed under the conditions defined in part a?
The hydrolysis of glucose-6-phosphate to glucose and phosphate is an important step in glycogen catabolism. The free glucose formed by this reaction is released intto the blood for transport to cells in need of energy glucose-6-phosphate +H20 right arrow Glucose/phosphate Mu M and the glucose and phosphate conventrations are 5mM each. Assume the appropriate enzyme is presnet to catalyze this reaction b. In which direction would you expect the reaction to proceed under the conditions defined in part a? Triangle G degree at 25 degrees when the conventration of glucose-6-phosphate is 20 Triangle G degree for this reaction is -3.3 kcal/mol at 25 degrees and pH 7.0 a. CalculateExplanation / Answer
Based on the given data,
Glucose-6-phosphate (Glu-6p) is hydrolyzed into glucose (Glu) and phosphate (Pi). The concentration of the (Glu-6p) is 20µM and (Glu) and (Pi) is 5 mM (5000 µM) at 25 degrees and pH 7.0 (physiological conditions).
The standard free energy change for the reaction is:
Glucose-6-phosphate +H20----> Glucose/phosphate Go = -13.8 kJ/mol
Q = [Glu][Pi]/[Glu-6p] = (5x 10-9)(5 x 10-6)/ (20 x 10-6)= 1.25 x 10-24
G =Go+ RT ln Q
= 13800 J/mol + (8.31 J/mol-K) (310 K) ln (1.25 x 10-24)
= -3254.74J/mol
or -3.25 kJ/mol
a) Thus, the Gfo for this reaction is -3.3 kcal/mol at 25 degrees and pH 7.0
b) The reaction will happen spontaneously under the conditions.
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