Table 3-5 Enzyme Concentrations Class Data Tube # Enzyme Concentration 1% 0.2 %
ID: 3521967 • Letter: T
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Table 3-5 Enzyme Concentrations Class Data Tube # Enzyme Concentration 1% 0.2 % 0.04 % 0.008 % Enzyme activity '1/T t. I lo 0.02 0.00 none Table 3-6 pH-Class Data Tube # | HCI Concentration Enzyme activity 'I/T pH 1- L03 0.2M 0.1M 0.05M 0.025M 0.013M 0.35 D.42 D.29 D.20 0.05 7 2.5D 2.78 10 Table 3-7 Temperatures- Class Data Tube # Temperature C Enzyme activity '1/T 0 6.07 0.51 0.27 12 13 14 15 16 5.S0 37.00 b.0D gA.GS O.3D 0.00 Plot enzyme activity (vertical axis) versus enzyme concentration (horizontal axis) on Figure 3-7, enzyme activity versus pH on Figure 3- temperature on Figure 3-9. 8, and enzyme activity versusExplanation / Answer
17.
Active site is the region of an enzyme where substrate molecules bind and undergo a chemical reaction.
As the pH changes, the active site of the enzyme progressively distorts and affects enzyme function.
18. Pepsin tolerates very low pH compared to most proteins. It is because of fact that pepsin is much more effective at breaking down protein when the pH is very low. Pepsin is shaped in such a way that it does not build up excessive positive charge at low pH, so it doesn't unfold easily at low pH.
19.The clarification rate of albumin will not change because at pH nearly 2 enzyme trypsin is inactive .
20. This change is caused as we know the enzyme activity is dependent on pH.Different enzymes have different ph for enzyme activity . Variations in pH cause changes in amino acids that can distort an enzyme into a less effective shape. Different enzymes have different optimal pH because they are designed to operate in different environments.like pepsin prefers the low pH -- about 2.0 -- associated with stomach acid.. Trypsin's optimum pH is about 8.So it won't affect albumin clarification.
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