When adding your DNA samples to the agarose gel, you will need to add loading dy

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When adding your DNA samples to the agarose gel, you will need to add loading dye to the samples. Loading dye contains:

glycerol, which helps the samples sink into the sample wells of the gel; and

tracking dyes so that you have a way of estimating how far the samples have travelled through the gel during electrophoresis.

The recipe for 6X loading dye is as follows:

0.25% bromophenol blue (w/v)

0.25% xylene cyanol (w/v)

30% glycerol in water (v/v)

11. You will be asked to make 20 ml of 10X loading dye. Using the information above, provide a recipe. (Important tip: Remember that you have already made 30ml of a 50% stock solution of glycerol. I recommend determining the concentration of glycerol required for 10X loading dye, then figuring out how much of your 50% glycerol stock will be needed to make 20ml of 10X loading dye.)

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When adding your DNA samples to the agarose gel, you will need to add loading dy

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When adding your DNA samples to the agarose gel, you will need to add loading dy

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