6.ELISA’s are both specificand sensitive. A. Explain why antibody specificityis

Question

6.ELISA’s are both specificand sensitive.

A. Explain why antibody specificityis crucial to the functioning of the ELISA test.

B. What component(s) of the ELISA provide sensitivity?

7, Direct ELISA is frequently used to test for the presence of bacterial toxins in the body fluids. Conversely, Indirect ELISA is more likely to be employed to test for HIV infection. Indirect ELISA can also be used (albeit infrequently) to test for infection by Chlamydia trachomatis, the causative agent of the sexually transmitted disease, chlamydia. Why is Indirect ELISA a better test for both HIV infection and Chlamydia than a Direct ELISA? (Hint: Chlamydia trachomatisis an obligate intracellular parasite).

Explanation / Answer

6.A. Antibodies are crucial in ELISA because they detect and quantity proteins and peptides. The specificity of assay relies on antigen antibody interaction. Highly specific antibodies are key to a successful ELISA.

B. The components that provide sensitivity are antibodies. Other components include buffer and substrate. ELISA uses stable immune complexes and provide the most effective sensitive detection.

7. Indirect ELISA is better because of presence of primary and secondary antibodies and in this, the secondary antibody binds to constant region of primary antibody and this the infection can be found out very quicky and easily.

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