Please help clearly explain this animal physiology question, thank you Question:

Question

Please help clearly explain this animal physiology question, thank you

Question: Because you want to test what you learned in lecture, you take the frogs from the Arctic that were at 2°C and place them at 25°C and isolate their LDH enzymes after a few hours. Will the substrate binding affinity of the LDH enzyme of Arctic frogs at 25°C (measured at 25°C) be higher or lower than the substrate binding affinity of the LDH enzyme of Arctic frogs at 2°C (measured at 2°C)? You leave these frogs at 25°C for a few weeks, will their Km increase or decrease? Explain your answer

Explanation / Answer

The activity of enzyme increases with increase in temprature but only upto a certain limit after which if temprature is increased it leads to protein denaturation and decrease in the activity of enzyme.in case of frog the temprature 25 degree centigrade is with in range so the affinity of the enzyme will be higher for substrate at 25 degree than at 2 degree

Km is the concentration of substrate which permits the enzyme to achieve half of Vmax.more the Km ,less is the affinity of the enzyme.as we have seen at 25 degree centigrade the affinutyais higher that means the Km is low.that is Km will decrease.that is less amount of substrate will be required to achieve half of Vmax

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