T e) Many DNA polymerases have nuclease activity,allowing the removal of mismatc
ID: 3516182 • Letter: T
Question
T e) Many DNA polymerases have nuclease activity,allowing the removal of mismatched bases f Translation begins near promoter sites and ends at terminator sites e) Prokaryotic mRNA processing in eukaryotic mRNA. undergoes posttranslational processing, but there is no postranslational h) The anticodon, consisting of three bases in transfer RNA, recognizes a complementary 3 base sequence in the mRNA called the codon. i) The genetic code is overlapping and non-degenerate 1) The restriction enzymes recognize cleavage sites of 4 to 8 nucleotides in length that are k) Fluorescence detection of oligonucleotide fragments produced by the dideoxy method requires 1) PCR cycles will amplify the target sequence (short strand) exponentially and the parent palindromi radioactive labeling of the nacleotides sequence (long strand) arithmetically m) If the concentration of salt in solution increases, the melting temperature of DNA decreases. n) As the percentage of the G-C base pairs increases, the melting temperature of DNA decreasers o) In the lung, the presence of higher concentration of O promotes the release of H and co, from hemoglobin. p) The affinity of hemoglobin for 0-1ncreases with increas ) The oxidation state of Fe in the functional myoglobin is+3. of the C02 concentration _ r) The acidic environment of an exercising muscle makes hemoglobin bind to O, more stromgly s) A single amino acid substitution in the B-chain of hemoglobin causes sickle-cell anemia. t) Myoglobin is an allosteric protein, whereas hemoglobin is not. u) The affinity of hemoglobin for Ois regulated by an organic phosphate (23-BPO), whereas the affinity of myoglobin for O is not. v) In the lung, the presence of higher concentration of O; promotes the release of H and CO,.Explanation / Answer
i)The genetic code is overlapping and non-degenerate- False, beause it is degenerative and non overlapping
j) The restriction enzymes recognize cleavage sites of 4 to 8 nucleotides in length that are palindromic-True
k) Fluorescence detection of oligonucleotide fragments produced by the dideoxy method requires radioactive labelling of neucleotides.- True
l) PCR cycles will amplify the target sequence (short strand) exponentially and the parent palindromi radioactive labeling of the nacleotides sequence (long strand) arithmetically-True
m) If the concentration of salt in solution increases, the melting temperature of DNA decreases. -False melting temperature increases.
n) As the percentage of the G-C base pairs increases, the melting temperature of DNA decreasers-False- melting point increases.
o) In the lung, the presence of higher concentration of O promotes the release of H and co2, from hemoglobin.-True
p) The affinity of hemoglobin for 02 increases with increase in CO2 cocnetration-True
q) The oxidation state of Fe in the functional myoglobin is+3._False. it is ferrous state +2 when it can bind with oxygen and is functional.
r) The acidic environment of an exercising muscle makes hemoglobin bind to O, more stromgly-False. Exercise causes increase in metabolism, temperature and acidity which leads to O2 dissociation and supply to muscle which are much in need of oxygen.
s) A single amino acid substitution in the B-chain of hemoglobin causes sickle-cell anemia.True
t) Myoglobin is an allosteric protein, whereas hemoglobin is not. False- Hemoglobin is also an allosteric protein
u) The affinity of hemoglobin for Ois regulated by an organic phosphate (23-BPO), whereas the affinity of myoglobin for O is not.-True because it has more affinity to oxygenated hemoglobin than to de oxygenated hemoglobin.
v) In the lung, the presence of higher concentration of O; promotes the release of H and CO,. -True.
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