Your colleague Prof Plum is studying the bacterial enzyme texase, which breaks d

Question

Your colleague Prof Plum is studying the bacterial enzyme texase, which breaks down the sugar texose. The protein sequence of the active site of texase is Met-Gly-Tyr-Ilu-Tyr-Tyr-Ala-Tyr-Gly. Prof Plum discovers a mutant form of bacteria that breaks down texose twice as fast. When he isolates the mutant texase gene, he discovers that the active site of this fast form has the sequence: Met-Gly-His-Ilu-Tyr-His-Ala-HisGly and the DNA sequence: AUG GGC UAC AUC UAU UAC GCC UAC GGA. Prof Plum does not know how to explain this result. Suggest a possible explanation.

Explanation / Answer

It should be noted that there is no difference in the number of amino acids in the wild type and mutated enzyme, texase.

Wild type - protein sequence - Met-Gly-Tyr-Ilu-Tyr-Tyr-Ala-Tyr-Gly

Mutated version - protein sequence - Met-Gly-His-Ilu-Tyr-His-Ala-His-Gly

Right DNA sequence for mutated version - AUG GGC CAU AUC UAU CAU GCC CAC GGA

Probable sequence for the wild type - AUG GGC UAU AUC UAU UAC GCC UAU GGA

Thus it is understood that there is base substitution mutation in the third amino acid sequence, sixth amino acid sequence, and in the penultimate amino acid sequence that has converted the tyrosine residue to histidine residue.

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