A curious student named Rengin, inspired by her Physiology course conducted an e

Question

A curious student named Rengin, inspired by her Physiology course conducted an experiment. She made an artificial cell containing an aqueous solution of 200 mM sucrose, 400 mM glucose, and 500 mM fructose. The membrane she used to make the cell was selectively permeable to water and to the monomer sugars glucose and fructose, but is completely impermeable to the disaccharide sucrose. She inserted her artificial cell in a beaker containing a different aqueous solution 400 mM sucrose, 600 mM glucose, 200 mM fructose. 200 mM sucrose 400 mM glucose 500 mM fructose 200 mM fructose 600 mM glucose 400 mM sucrose Answer the following Questions and justify your answer. 1. Which solute(s) will exhibit a net diffusion into the cell? 2. Which solute(s) will exhibit a net diffusion out of the cell? 3. In which direction will there be a net osmotic movement of water? 4.After the cell is placed into the beaker, will the cell become more flaccid or turgid? Explain.

Explanation / Answer

1. Glucose will diffuse into the cell as it has higher concentration (600 M)in surrounding then inside(200M).

2. Fructose will move out of cell from region of 500mM to 200 mM.

3. As concentration of all salts together is higher outside so net movement of water will be from outside to inside the cell .

4. It will become flaccid due to exosmosis of water from inside to outside . As salt is more outside . So there is less water outside than compared to inside of the cell. This causes movement of water from it's high concentration ( inside) to low concentration( outside) making it flaccid.

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