BIOL 2117L/Microbiology Laboratory Dichotomous Key for Haemolysis (Bishop) Haemo
ID: 3514169 • Letter: B
Question
BIOL 2117L/Microbiology Laboratory Dichotomous Key for Haemolysis (Bishop) Haemolysis (hemolysis) testing is often used for distinguishing Gram (+) bacterial from one another, specifically the Streptococci sp, Staphylococci sp, and Enterococci sp. Bacterial alpha-hemolysis results in the oxidation of iron contained within hemoglobin, which will leave a green pigmentation in the BA (Blood Agar) surrounding a alpha-hemolytic bacterial colony. Bacterial beta-hemolysis results in the complete destruction of red blood cells, which will leave a cleared (or loss of color) within the BA surrounding a beta-hemolytic bacterial colony Bacterial gamma-hemolysis (majority) does not result in oxidation or hemolysis on BA, leaving an unchanged appearance around the bacterial colony. Potential Gram(+)Bacteria Streptococcus agalactiae Streptococcus pneumoniae Mycobacterium phlei Staphyloccus aureus Staphylococcus epidermitis Streptococcus pyogenes *Streptococcus salivarius Enterococcus faecalis Using the bacterial species listed above, construct a dichotomous key to separate the species from one another according to hemolytic reaction on BA (next page/over).Explanation / Answer
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