7 Th solation. One method, called frequency sound to disrupt the cell plasma mem

Question

7 Th solation. One method, called frequency sound to disrupt the cell plasma membrane ruption or a cell is necessary to release its organelles and contents for subsequent uses ultrabigh A) tomography B) cpitope ta sonication ation (this cell type) which quickly predominate over the other cell the proper space and nutrients needed to grow, these cells can divide about 50 times, 8. Primary cultures contain types. Given such that after 50 doubling a. fibroblasts; 2500 cells s, an original culture of 100 cells would become bfibroblasts; 1.1 × 1017 c. epithelial cells; 2500 d. epithelial cells; 4.5 x 10 small tumor is excised from a patient's body. The pathologist wants to examine the number, e, and arrangement of cells within the tumor. The best technique to use would be a. DIC microscopy b. phase contrast microscopy. c. b right-field microscopy after fixation, sectioning, and staining d. fluorescence microscopy. 10. Ultracentrifuges allow cell biologists to isolate mitochondria from lysosomes based on organelle differences in: a. isoelectric point. b, ionic composition. Equilibrium density

Explanation / Answer

7)C)SONICATION

cell membrane disrution in sonication is directed by ultrs-sound induced cavitation

the method uses pulse,high frequency sound waves to agitate and lyse cell plasma membrane

8)b) fibroblasts

fibroblasts divide 50 times before they cease growth.when initially there are 100 cells then after 50 doublings it becomes:100X2 raised to 50 times gives approx 1.1X10 raised to 17.

9)c)bright field microscopy, after fixation sectioning and staining

tumor cells can be best studied under a bright field of a microcsope after methods of fixation on a slide, sectioning of the specimen and staining of the specimen

10)c)equilibrium density

isolation of mitochondria from lysosomes is possible by the above method

it is a type of sedimentaion method in which molecule of high density sediments at the bottom

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