In newly formed neural tube, the intermediate region close to the sulcus limitan

Question

In newly formed neural tube, the intermediate region close to the sulcus limitans, has been not exposed to molecules that induce ventral or dorsal characteristics. You perform explant experiments in which you take intermediate neural tube (host tissue) and culture them with donor tissue explants that induce specific spinal cord markers in the host explant.

1. The induced host tissue express Shh following transplant.

a. What donor tissue have you explanted next to the host tissue, what signal is released to induce this marker, and what cell type has been induced? Briefly explain your reasoning.

b. What other cell types do you expect to observe? Briefly explain your reasoning.

2. The induced host neural tube expresses HB9. What donor tissue have you explanted next to the host tissue, what signal is released to induce this marker, and what cell type has been induced? Explain your reasoning.

3. How does the signal in parts A and B generate neuronal diversity in the ventral spinal cord? Use only 3 sentences.

4. The host animal is transgenically modified so that Shh irreversibly binds to the receptor. What do you expect to observe with the experiment in Part A?

5. The host animal is transgenically modified so that Pax6 and Pax7 do not respond to Shh signaling. What do you expect to observe with the experiment in part a?

Explanation / Answer

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