13. You are a performing an experiment on cheek cells. You swab your mouth and p
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Question
13. You are a performing an experiment on cheek cells. You swab your mouth and place them on a microscope slide. Then you drop an unidentified solution onto the cells to view them better under the microscope. When you look at the slide, you notice that the cells are no longer visible but see a lot of debris. What type of solution did you use? Explain what may have happened to the cells. 14. Briefly state the stages of cell division and what occurs at each step. Be sure to include the following key words (equatorial plate, mitotic spindles, cleavage furrow).
Explanation / Answer
13.Experiment on Cheek cells :
When performing an Experiment with Cheek cells by taking a swab from the mouth mucosal membrane and placing it in a glass slide, when we add a solution such as GRAM'S STRAIN or ZEIHL-NEELSON STRAIN,and when examined under the Microscope, Cells are no longer found.
We can find only Debris called as INHIBITED PATHOGENS.The strain ( inhibitory substance) does not kill the unwanted organism .But it merely suppresses or retards its growth on that medium.When this debris is subjected to sub-culture using another medium, it will result in further suppression of growth or in the absence of inhibitory substances may lead to the resumption of the growth of the pathogens.
The solution used is GRAM'S STRAIN .
The cells are SUPPRESSED in its virulence and INHIBITED.
14.STAGES OF CELL DIVISION :
A Matured cell divides into two daughter cells. Body cells of all animals and plant cells undergo a cell division called Mitosis. This involves changes in the structure of the chromosomes ,but without any change in the chromosomal number.
The germinal epithelial cells of animals undergo Meiosis cell division ,involving changes in the structure and number of chromosomes.
Mitotic cell division :
It is studied under 4 sub-divisions.
1.prophase
2.Metaphase
3.Anaphase
4.Telophase
1.Prophase :
The bivalent chromosomes gets shortened .The centrioles form asters and move into the poles.The nucleolus and nuclear membrane disappear.
2.Metaphase:
Chromosomes ,each consisting of two chromatids held together by a centriomere are arranged at the equator (equitorial plate )of the spindle fibres.The centriomeres are attached with the spindle fibres.
3.Anaphase:
The centriomeres divide into two ( cleavage ) and the two chromatids separate and now they are called as Daughter chromosomes or new chromosomes.The daughter chromosomes move towards the opposite poles.
4.Telophase:
The haploid set at the two poles coil to form chromatin material.The nuclear membrane and nucleolus reappear.Thus two daughter nuclei are formed.
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