Apply your knowledge of transcription and translation to answer this three-part

Question

Apply your knowledge of transcription and translation to answer this three-part question: Given the following template strand sequence for a prokaryotic gene, determine the sequence of the mRNA that would be produced by RNA polymerase. a. (3 marks) 5'-TGGCCAATGTACCCTTAGAATGACAGCATTCGCTAA-3 Using the Genetic Code table provided below, translate this mRNA into the peptide that would be produced from it. Indicate the amino and carboxyl termini of your protein. b. (5 marks) Phe Ser Tyr Cys C Leu Ser STOP STOP A Leu Ser STOP TrpG C Leu Pro His Arg U U Phe Ser Tyr Cys Pheser STO Leu Pro His Arg C Leu Pro Gin Arg A Gin ArU Leu Pro Gin Arg G Asn ser A A lle Thr Asn SerU lle Thr Asn SerC lle Thr Lys Arg A Met Thr Lys ArgG Asn Arg G G|Val Ala Asp Gly |U| Val Ala Asp Gly C Val Ala Glu GlyA Val Ala Glu Gly G c. What would happen to the protein if the 10th residue in the DNA sequence (from (1 mark the 5' end) were mutated from T to C?

Explanation / Answer

Ans- a- 5'-TGGCCAATGTACCCTTAGAATGACAGCATTCGCTAA-3'

3'-ACCGGUUACAUGGGAAUCUUACUGUCGUAA______-5'

B ans- thr-gly-tyr-met-arg-Ile-leu-leu-ser-stop-______after stop codon will not transcribe template DNA.

C ans-if the 10 th residue of DNA will change from the 5' end and mutated from T to C the amino acid sequence change and code for a different protein.(TAC convert into CAC and the mRNA sequence will be GUG this code for valine.

Thr-gly-Tyr-val-arg-Ile-leu-leu-set-stop

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