REVIEW QUESTIONS FOR AP/ CAP LAB 1. What is an action potential (AP? To anawer t
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REVIEW QUESTIONS FOR AP/ CAP LAB 1. What is an action potential (AP? To anawer this question you neod to know a. The resting membrane polarity. b. Why is there an electrical potential difference across the membrane c. The two major+ions involved.No d. The source of the-charges. e. The two types of channels found in the membrane of nerve and muscle tissoes f. When these channels open and closc. &. Why the channels open and close. h. Why the ions flow across the membrane i. What causes depolarization. j. What causes repolarization. k. What is the Na-K pamp: which direction it carries Na t; which direcztion it carries K I. The source of energy to drive the Na-K pump. 2. You should have a geoeral idea about: (Ak it yos ane not murm.) a Location of frog sciatic nerve. b. Location of your sciatic nerve. c. Be able to make a sketch of the cross section of a sciatic nerve - label nerve fibers (axons&dendrites) d. Know that there are hundreds of nerve fibers in the sciatic nerve. . That all sizes of nerve fibers are present (oype A, B, and C) f. Know the anatomical and physiological differeaces between A, B, & C neurons gmponductionDosentil (CAP), O2; Lan 3. Know the difference between an action potential (AP) and a aBe able to explain why an AP is differeat in appearance from a CAP·S" senqe-in.) b, what is a biphasic CAP? why is it biphasic? Draw a biphasic CAP. c. Explain how a CAP is recorded. (Disouss locasios of electrodes) d. What is a monophasic AP? Why is it monophasic? Draw a monophasic AP. e. Explain how an AP is recorded. Disces locasion of electrodes) 205 Lab-7 page 7Explanation / Answer
Answer
1.
The changes in membrane potential to positive charge and produce the action. The potential require getting an action in muscle or nerves is called action potential. The resting membrane potential is -70mV.
1A.
The resting membrane potential or electrical polarity is from -70 mV to -90 mV. There must be an equilibrium between potassium and sodium ion inside and outside of cell respectively.
1B.
The cell membrane maintaine, the difference ions concentration across the membrane and permeability of important ions. The unequal concentration of ions provides electrical charge to the membrane.
[Na+]ECF = 150mEq/L, [Na+]ICF = 15 mEq/L,
[K+]ECF = 5 mEq/L, [K+]ICF = 150mEq/L,
1C.
The two major ions involved are Sodium ions (outside the cell) and potassium ions (inside the cell)
1D.
Chloride ions and different types of proteins inside the cell and are located near to phospholipids membrane of the cell.
1E.
The two types of channels found in nerve and muscle tissue are Voltage-gated channels and ligand-gated channels.
Example of voltage-gated channels is Sodium channels, potassium channels, calcium channels, and chloride channels.
Examples Ligand-gated channels are GABA and glycine-mediated channels.
1F.
Voltage-gated channels open when the change in the voltage of membrane due action potential development. The channels are closed when the equilibrium occurs or after removal of the action potential.
1G.
The ion channels are opened due to a specific stimulus that changes the voltage of membrane and stress and some ligand binding. Channels are closed upon removal of the stimulus.
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