Which DNA sequences must a synthetic gene contain in order for the gene to be in

Question

Which DNA sequences must a synthetic gene contain in order for the gene to be inserted into the expression plasmid?

Origin of replication.

Restriction enzyme sites.

DNA ligase sites.

Ribosome binding site.

Lac operator.

Question 2

Select one answer.

10 points

Which of the RNA molecules brings the new amino acids to the growing protein chain?

mRNA

tRNA

rRNA

all of the above

Question 3

Select one answer.

10 points

Which of the following DNA sequences is a recognition site for restriction endonucleases:

AATT

ATTA

TTTT

AAAA

TAAT

Question 4

Select one answer.

10 points

Is the following statement true of prokaryotic cells, eukaryotic cells or both? One gene can encode multiple different proteins (different in amino acid sequence).

Prokaryotic

Eukaryotic

Both prokaryotic and eukaryotic

Question 5

Select one answer.

10 points

Assuming that the genes required for Isoleucine (an amino acid) biosynthesis are contained within an operon. If the cell is grown in the presence of high amounts of isoleucine you would expect to find:

The repressor protein to bind to the operator, preventing transcription.

The repressor protein to bind to the operator, allowing transcription.

The repressor protein released from the operator, allowing transcription.

The repressor protein released from the operator, preventing transcription.

Question 6

Select one answer.

10 points

The tRNA with UAU as the anticodon would be attached to which amino acid?

tryrosine

isoleucine

leucine

serine

Question 7

Select one answer.

10 points

Which of these is not used in DNA replication?

RNA polymerase

DNA polymerase

Helicase

Primer

Question 8

Select one answer.

10 points

A gene codes for a protein that is 500 amino acids long. Which of the following mutations is likely to be the most deleterious?

a missense mutation at the 490th codon

a missense mutation at the fifth codon

a nonsense mutation at the fifth codon

a nonsense mutation at the 490th codon

Question 9

Select one answer.

10 points

A codon in the middle of an mRNA strand was mutated from UGU to UGA. What type of mutation is this?

nonsense

missense

silent

frame shift

Question 10

Select one answer.

10 points

The correct decoding of the following encrypted message: PQRMNO is?

Key(MNO = h PQR = i)

ih

hi

Question 11

Select one answer.

10 points

Separation of the original DNA strands is due to the action of:

polymerase

RNA primers

single stranded binding protein

helicase

Question 12

Select one answer.

10 points

The DNA sequence of the expression plasmid adjacent to the ribosome binding site is:

---GGATCCATCTGCGAATTC----

---CCTAGGTAGACGCTTAAG---

It contains a BamH1 site (G^GATCC) and a EcoR1 site (G^AATTC). In order for the synthetic gene to be inserted between these two sites, it should be constructed to have which restriction sites at its beginning and at its end?

BamH1, followed by BamH1.

BamH1, followed by Eco R1.

EcoR1, followed by BamH1.

EcoR1, followed by EcoR1.

Question 13

Select one answer.

10 points

Which of the following terms refers to a region of a chromosome that has a specifiic function?

Genotype

Allele

Gene

DNA

Question 14

Select one answer.

10 points

In most bacterial operons, such as the lac operon, which of the following DNA segments is responsible for controlling whether the operon is off or on?

Operator

Repressor

lacY gene.

Promoter

Question 15

Select one answer.

10 points

In an expression plasmid, the origin of replication: _________________

allows for growth of plasmid containing bacteria in the presence of antibiotics.

Is the position where the base number begins at 1.

is responsible for the replication of mRNA.

allows the bacteria to duplicate the plasmid.

Explanation / Answer

1)

The Restriction enzyme sites should be present in order for the gene to be inserted into the expression plasmid. Thus, the correct option is (b).

2)

The tRNA molecules bring the new amino acids to the growing protein chain. Thus, the correct option is (b).

3)

AATT

4)

Prokaryotic

5)

The repressor protein to bind to the operator, preventing transcription

6)

Isoleucine
7)

RNA polymerase

8)

A nonsense mutation at the fifth codon

9)

Nonsense

10)

ih

11)

Helicase

12)

BamH1, followed by Eco R1

13)

Allele

14)

Operator

15)

In an expression plasmid, the origin of replication: Is the position where the base number begins at 1.

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