F Effect of Osmolarity of \"Plasma\" on Red Blood Cells Draw a red blood cell fr

Question

F Effect of Osmolarity of "Plasma" on Red Blood Cells Draw a red blood cell from each slide. (3 pts) Hypotonic saline Isotonic saline Hypertonic saline I. How would water intoxication affect the size and the shape of red blood cells? (2 pts) 2· The isotonic solution that you used was 0.9% NaCl, what was the osmolarity of this solution? 3. The osmolarity of the hypertonic solution was 1.7 OsM. How many grams of NaCI were used to make 500 mL of this solution? (2 pts) 4. Name one factor that could cause an elevated WBC. (1 pt) 5. Name one factor that could cause an elevated RBC. (1 pt)

Explanation / Answer

1. when water enter in cell from hypotonic solution it swells and may burst. when water comes out of a cell in hypertonic solution then cell shrinks with irregular shapes.

2. mol. weight of NaCl = 58.44

given here, 0.9% NaCl , so no. of moles of NaCl= 0.9/ 58.44     x     1000/100 =0.154 moles/litre

NaCl dissociate into Na + and Cl -

so no. of osmoles in one mole of NaCl is 2.

here we have 0.154 moles/litre so 2 x 0.154 = 0.308 Osmol/litre

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