Imagine you have just sequenced a small bacterial mRNA: 5’ – UCGAUGGGCUGAUGUUUGG

ID: 3478439 • Letter: I

Question

Imagine you have just sequenced a small bacterial mRNA:

5’ – UCGAUGGGCUGAUGUUUGGCACGGCUCAGUCCCUGCCAAGUUCGGGAA GGCCUCUCGAAGAGCGAGUACACAGGCCCUCUUGCUUUUAGCGCUGGAUG-3’

1. How many amino acids (including fMet) are present in the most probable (longest) polypeptide encoded by this mRNA?

2. Which amino acid is located at the C-terminus of this polypeptide?

3.What is the 3’ UTR sequence in this mRNA?

4.How many tryptophan (trp) molecules are present in the polypeptide product of this mRNA?

5. How many leucine (leu) molecules are present in the polypeptide product of this mRNA?

1. How many amino acids (including fMet) are present in the most probable (longest) polypeptide encoded by this mRNA?

Out of the three frames, frame 3 encodes most probable polypeptide.

AUG UUU GGC ACG GCU CAG UCC CUG CCA AGU UCG GGA AGG CCU CUC GAA GAG CGA GUA CAC AGG CCC UCU UGC UUU UAG

Met Phe Gly Thr Ala Gln Ser Leu Pro Ser Ser Gly Arg Pro Leu Glu Glu Arg Val His Arg Pro Ser Cys Phe

2. Which amino acid is located at the C-terminus of this polypeptide?
Phe

3.What is the 3’ UTR sequence in this mRNA?
UCGAUGGGCUG

4.How many tryptophan (trp) molecules are present in the polypeptide product of this mRNA?
0

5. 5. How many leucine (leu) molecules are present in the polypeptide product of this mRNA?
2. (Bold and underlined in the above polypeptide chain)

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