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1. what is the purpose of the exonuclease I enzyme? 2. why was the discovery of

ID: 34763 • Letter: 1

Question

1. what is the purpose of the exonuclease I enzyme?

2. why was the discovery of Taq DNA polymerase important for the development of PCR?

3. The protein GAPDH is necessary for cellular function and is highy conserved between organism.Why is it probable that proteins needed for cell survival will be very similar (highly conserved) in many different organisms?

4. what is a consensus sequence?

5. what would be the consensus sequence for the following aligned sequences?
ATTGCTTC
AATGCTAC
ATTCCTAC
ATTGCTAC

Explanation / Answer

1. Exonucleases are enzymes that work by cleaving nucleotides one at a time from the end (exo) of a polynucleotide chain. A hydrolyzingreaction that breaks phosphodiester bonds at either the 3