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While viewing a microscope slide with the scanning objective lens the total magi

ID: 3474842 • Letter: W

Question

While viewing a microscope slide with the scanning objective lens the total maginification is 40X and the diameter of the field of view is 5.0mm. Switching to the low-power objective lens will increase the total magnification to 100X. Calculate the diameter of the field of view at this higher magnification. While viewing a microscope slide with the scanning objective lens the total maginification is 40X and the diameter of the field of view is 5.0mm. Switching to the low-power objective lens will increase the total magnification to 100X. Calculate the diameter of the field of view at this higher magnification.

Explanation / Answer

of the field of view is inversely proportional to the total magnification.

Hence the equation is:

Magnification of Scanning objective lens (A)= 1/Diameter of field of view (A)

Magnification of low power objective lens (B)= 1/Diameter of field of view (B)

In order to calculate the diameter of field of view for low power lens, the following equation is applied.

Magnification of A/Magnification of B = Diameter of B/Diameter of A

Diameter of B= Magnification of A X Diameter of A/ Magnification of B

                         = 5 mm X 40X/ 100X= 200 mm/100= 2 mm

Hence, the diameter of field of view for low –power objective is 2mm