Before placing a slide on the microscope stage, you observe the tissue section w

Question

Before placing a slide on the microscope stage, you observe the tissue section with the naked eye and notice that it has the following shape: Next, you view the slide under low magnification so that the entire tissue section is visible in the field of view. In the space below, draw the tissue section as it will appear under your microscope. Explain what is meant by depth of field. While viewing a microscope slide with the scanning objective lens, the total magnification is 40X and the diameter of the field of view is 5.0 mm. Switching to the low-power objective lens will increase the total magnification to 100X. Calculate the diameter of field of view at this higher magnification.

Explanation / Answer

15) Depth of the field is defined as the distance between nearest object plane in focus to that of the farthest plane simultaneously in focus. Depth of the field decreases with magnification.

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